What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. #ABS Plus) 4s3 ™ Semi-Automatic Sheet Heat Sealer (Brooks Life Sciences cat. plates < 100 amol (384-well) 1 0200-7018. Embla washers are durable and easy to maintain. and 384-well microplates at a selected wavelength for a single point in time (endpoint), over a specified period of time (kinetic), or over a selected wave-length range (spectral scan). Select Consumables. The same measurement settings can be used for subsequent plates as long as the volumes, tracer and concentrations remain the same. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. 58% for the detection module and 2. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. and the A1 corner of the plate adapter (Figure 1-2). 25mm: Plate height:Absorbance was measured at 490 nm with SpectraMax Plus 384 plate reader (Molecular Devices, San Jose, CA). Temperature Control: Ambient +5C up to 42C. We would like to show you a description here but the site won’t allow us. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. 48 ± 0. for the SpectraMax Multi-Mode Microp late Reader, as well as all other Molecular Devices' microplate readers. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. 6-, 12-, 24-, 48-, 96- and 384-well plates: Tablet PC: Windows® 8. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. NanoQuant plate. The FilterMax F5 can read 6, 12, 24, 48, 96, 384, 1536-well. Well scans from the Gemini EM Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM/Ethidium Homodimer-1) assay kit. Important things to consider when purchasing a multimode microplate reader. #ID5) HTRF Detection System (Molecular Devices cat. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Values are averages from 5 clean plates, each filled with 200 µL water/well. High-throughput screening (HTS) can efficiently assay multiple discrete biological reactions using multi-well microplates. VIS absorbance detection. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. † Bottom mounting for SpectraMax Paradigm Readers only. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. Both formulations utilize Molecular Devices proprietary quench technology to enhance signal windows and yield acceptable Z-scores to screen a variety of targets, including TRP, ligand-, cyclic nucleotide- and voltage-gated channels. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. 6-, 12-, 24-, 48-, 96-, and 384-well microplates; Extended research windows available; Temperature control; Returnable samples; Repeatable experiments;The SpectraMax ABS Plus Microplate Reader can run both cuvette-based and microplate based reader applications on the same instrument. Catalog number: BC1384W. x. 1 or 10 Tablet PC with USB connection to instrument: Read Speed: 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes: Automation Compatibility: Hardware and software accessible to third-party hardware/software control, including LIMS. Two different agonists, acetylcholine and carbachol, were tested. x. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent. 375 pM 1 FP Fluorescence Polarization detection for fluorescein- or rhodamine-like labels Using specific LED and EX/EM filters for 6- to 1536-well plates 0200-7009POS 0200-7010POS Fluorescein FP EX 485 nm, EM 535P and 535S nm Rhodamine FP EX 535 nm, EM 595P and 595S nm 96–1. Suggested plates are black. Fluorometers also vary in the size of samples they accept, with some using very small sample sizes to conserve precious sample materials (down to 0. Outer wells aligned for proper viewing with FLIPR. Suitable for use in BMG lab tech, Molecular Devices, Promega Glomax and other plate readers; Suitable for adhesive and heat sealing: our Moisture Barrier Seal 384. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. 2, prime the machine with CellTiter-Glo ® 2. Microplates, cuvettes (via adapter) Detectors. Includes PC and software. x. A2000 Full Wavelength Microplate Reader, 6 ~ 384 Well Plate, SHANGHAI PEIOU ANALYSIS INSTRUMENT CO. The SpectraTest validation. . Bioz Stars score: 86/100, based on 9 PubMed citations. 5577. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 340 PC384. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. FLIPR Calcium 4 Assay Kit (Molecular Devices, San Jose, CA, USA). 5 ng per well. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. It comes standard with 4 wash/rinse bottles and 1 waste bottle for maximum washing flexibility. 020 fmol 0. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96-. M5. Cell viability는 세포막 Integrity 또는. Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). This chapter is an introductory overview of the most commonly used assay methods to estimate the number of viable cells in multi-well plates. With optimized reagents, option to operate the readers. The results above revealed excellent detection limits and dynamic range in all three Molecular Devices microplate luminometers. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. These robust readers have been placed in labs from Antarctica to the. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 3. We would like to show you a description here but the site won’t allow us. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. M2. Therefore, it is recommended that the pipettor height to be set to correspond to the fluid volume in the cell plate prior to compound addition. x. The system provides automation for up to 50 microplates for easy walkaway automation. Related products. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. Next generation SpectraMax® absorbance microplate readers. The plate reader can measure multiple readouts, including UV and. x. Microplate readers provide the ideal measurement platform for the high-throughput. Made from polypropylene with conical well profiles to maximize recovery and allow working with sample volumes as low as 5 µL. Fluorescent reading data were analyzed by non-linear fitting (GraphPad Prism, GraphPad Software Inc. Dimensions 128 x 86 x 15 mm. For a higher . For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. 3% in white 96- and 384-well microplates < 3 x 10-5 (white plates) < 3 x. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. 1. Nanoracks’ Plate-Reader-2 (2nd generation) is a reconfigured Molecular Devices SpectraMax M5e – one of the most reliable, durable, feature-rich microplate readers on the market. An IL-22 standard curve was prepared and a sandwich ELISA performed using the MultiWash+ plate washer in strip mode to wash the wells. SLS & DLS: 2 µL quartz cuvettes 45 µL quartz cuvettes 4 mL generic glass cuvette DLS only:Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. ". molecular interaction studies, and nucleic acid quantification, as well. All stand-alone instrument functions can be controlled using the software. Application Area: Spectrophotometer for molecular biology. Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. Round well design with a clear flat bottom 2. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. A dependable washer is quiet, easy to clean and leaves no residue, preventing contamination and reducing background. The ImageXpress® Pico Automated Cell Imaging System is more than a digital microscope, combining high-resolution imaging with powerful analysis. The plate reader consisted of a large-area. the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. The plate was mixed gently for 2 minutes on a plate shaker and then allowed to incubate at room temperature for 10 minutes to stabilize luminescence. Artel MVS Bundle DM Std/800TSNB Reader Details. For more sample throughput, the system can384 Well Plate, supplied by Molecular Devices LLC, used in various techniques. Versa Max. Explore the best practices for running absorbance-based assays how Molecular Devices absorbance readers can be utilized to optimize said absorbance-based assays. 2. Industry standard disposable (choose from recommended list). x. Read one sample or up to 384. 0 mP 384–1. Read one sample or up to 384 samples in a single plate by using any standard cuvette, or 96- or 384-well microplate. A2000 Full Wavelength Microplate Reader, 6 ~ 384 Well Plate, SHANGHAI PEIOU ANALYSIS INSTRUMENT CO. x. or use the modified protocol below for the generation of NPCs (see Note 1). It is also important to note that organoid growth patterns are relatively consistent from 24- to 384-well plates. 384 well plate (Molecular Devices LLC) About; News;Disposable Pin Tool - 96 / 384 Head: Fixed Pin Tool - 384 Head: Grippable Pin Tool - 96 / 384 Head: Magnetic Pin Tool - 96 / 384 / 1536 Head: LUMINESCENCE MICROPLATE READING: Molecular Devices: SpectraMax L: Thermo Fisher Scientific: Luminoskan Ascent: MICROPLATE CYTOMETRY: Nexcelom Bioscience: Celigo S Cell Imaging. 384 Well Optically Clear Tissue Culture Plate: Plate length: 127. (That difference comes at a cost, Amouretti explains, as higher plate densities require better optics and more sensitive detectors. the iD5 reader’s filter system. #6590-0144; includes Enhanced TRF Module, excitation and emission filters). • 11/10 • Printed in USA • PN: 0120-1525. 100 fmol 1536-well (8 µL) 0. DynaPro ™ Plate Reader III. USA. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 96-1536. Rating: 3. ZERO BIAS - scores, article reviews, protocol conditions and more. #W50-100). Category 2 and 3 microplate readers use gradient heating as well. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. By measuring optical pathlength, it. SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. #S1207. 과 같은 Parameter를 검사하여 평가할 수 있습니다. Documentation Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. No. #0200-6263) UltraVision™ Plate 384 (Brooks Life Sciences cat. Abs). x. San Jose, Calif. (1536-well plate) 2 µL (quartz cuvette), 4 µL (disposable cuvette). Temperature Control: ambient from +4 to 45C. The purposes of the study were: 1) to determine the optimal wavelength settings for three cell lines. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates andThe Raman plate reader was designed to simultaneously observe the Raman spectrum of 192 analytes loaded in 192 wells on a standard 384-well plate. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofThe plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Also refer to this Multimode Reader Comparison. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. The screw remains inside the instrument. 7. All stand-alone instrument functions can be controlled using the software. 6-384 (96- and 384-well with pipetting) 96-well microplates. F3/F5 Multi-Mode Microplate ReadersBoth reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. Reads 96-well microplates in 5 seconds; Reads 384-well microplates in 16 seconds, Monochromator, tunable 1. SpectraMax® iD5 Multi-Mode Microplate Readers,6 to 384-Well Microplates, Molecular Devices, LLC (CAT#: STEM-LE-0504-LC) Product Keyword. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Microarray scanners for one-fluor to multi-fluor [email protected] and Download Molecular Devices SPECTRAmax PLUS 384 operator's manual online. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. Synergy NEO multi-mode plate reader: BioTek: n/a: EL406 plate washer and bulk dispenser with wide-bore tip 10 μL cassette: BioTek: n/a:. of samples in both 96- and 384-well microplates or in a cuvette at a selected wavelength for a single point in time (endpoint), over a specified period of time (kinetic), or over a selected wavelength range (spectral scan. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96- or 384-well head. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Turn on Plate Reader and wait for it to complete its start-up routine. Multichannel processes: Costar 96-well (3300) and Greiner 384-well (781182/781185) microplates are validated for multichannel processes. Molecular Devices SpectraMax Plus 384 Absorbance Reader Details. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modes<2 fg/well for firefly luciferase in 96- and 384- well top read. Washers configured for both 96- and 384-well plates. $542. Unmatched throughput FLIPR Membrane Potential Assay Kits allow the elimination of wash steps and much shorter read times on the instrument. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax Plus 384 Microplate Reader (Molecular Devices cat. The Embla 96/384 Well Washer accepts 96-and 384-well format microplates and is ideal. Figure 2. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. To address this concern, Molecular Devices offers the FlexStation® 3 Multi-Mode Microplate Reader. The assay methods covered include. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. ability to use as little as 2˜µL samples with 24- or 64-well plates. Uncontrolled Copy for Training and Reference Purposes Only . for ELISAs and most cell-based assays. 635. (40 µl maximum) Clear, white, or black. 384-well TC plate, Poly-d-Lysine, Flat. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andSupplier: Molecular Devices. 3 µl). This eliminates the need for standard curves, and Flexibility to read a variety of plate formats. For more sample throughput, the system canThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Select Start>Control panel>Device Manager. Important things to consider when purchasing a multimode microplate reader. #R8190 (10) vials of component A*Instruments with a 96- or 384-well format will accommodate the most commonly used plates. Or scan up to 384 wells at any time to determine the best assay conditions. " A versatile UV/VIS spectrophotometer for both 96- and 384-well. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. xABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. Summary of Contents for Molecular Devices SpectraMax M2. • The FlexStation™ II³⁸⁴ instrument is designed for 96- and 384-well microplates. For biotherapeutic development, the DynaPro Plate Reader is a comprehensive solution for stability evaluation. Plate readers, also known as. Documentation:Molecular Devices microplate readers. More Product Information. 384-WELL SBS STERILE MICROPLATE. 003 fmol 0. It features a modular design that allows users to add or change detection cartridges according to their needs. Plate stacker and robot integration The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Made from polypropylene with conical well profiles to. The Standard Thermo Scientific 384-well plate, white, is compatible with the majority of 384 block PCR and sequencing instruments. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Plate dimensions: X6001, X6004, X6011, X7001, X7007, X7020, X7022, X5101, X6026. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. 0200-6262. 1 OD at 230 nm. x. The equation that allows one to calculate absorbance from % transmittance is. Plates were read in the SpectraMax M5 microplate reader at 485ex/515em with bottom read. Get Quote . VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Add 100 µl (96-well plate) or 25 µl (384-well plate) of cell suspension to each well of poly-D-lysine coated plates. 50 μL total well volume, with recommended working volume of 5-40 μL. 96/384-well plate formats. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Here, we show that Molecular Devices microplate readers can easily and noninvasively measure fluorescent proteins in living cells. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. Outer wells are aligned for proper viewing with FLIPR. The opaque walls prevent stray light from being detected from neighbouring. 2. Molecular Devices 1311 Orleans Drive Sunnyvale, California 94089 Telephone: +1-408. The SpectraMax 190 reader can be integrated with Molecular . Guaranteed sensitivity: < 100 amol of biotinylated-LCK-P peptide, 25µL/well in a 384-well plate. 100 ˜L (96-well) or 25 ˜L (384-well) of Loading Buffer was added to each assay well. • Easy and gentle immobilization of biotin. EPOCH2 Plate Reader/Spectrophotometer. 6-, 12-, 24-, 48-, 96- and 384-well plates: Tablet PC: Windows® 8. #PLUS 384) UV-transparent 96-well microplates (Corning cat. The patented quench technology is offered to drug discovery and life science researchers exclusively by Molecular Devices,. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. 315 can't find zero order. )Step 4: Incubate at room temperature for 1 hour, protected from light. 1 pM 1536-well: 0. The washer has a small footprint and is vacuum and pressure free, The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. Molecular Devices 大中华区 Email: info. If no reference reading is taken, the instrument uses the I 0 values stored in the NVRAM (non-volatile memory) of the. flat meniscus), but is approx 15 mL. The plate reader optics are tunable for. Instrument settings are listed in Table 1. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Incubate the plates at room temperature for 20 min before reading plate luminescence using a Paradigm plate reader (Molecular Devices) (see Note 14). Dual injectors for kinetic assays. DynaPro ™ Plate Reader III. Molecular Devices has developed the FLIPR Membrane Potential Assay Kits to maximize. FlexIII. Contact Us Phone: +1-800-635-5577 Web: Email: [email protected] nM Z Factor at EC. Extended linear dynamic range. 20 plates/hour (2-minute read time) 96-well 1,920 data points/hour 384-well 7,680 data points/hour 1536-well 30,720 data points/hour Bar code manual mode Keyboard entry Bar code remote mode Short or long side Temperature control Ambient +5°C to 40°C General specifications Dimensions (in. ‘StimB’ is Stimulation Buffer, included in the IP-One Gq kit. The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-wellThe FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. In the M2, M5 and Plus. x. com Check our website for a current listing of worldwide distributors. Supplier: Molecular Devices. plates < 100 amol (384-well) 1 0200-7019. Tunable wavelength selection / monochromator. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. by a photomultiplier tube (PMT) or charge-coupled device (CCD) present within the plate reader. 2 OD at 200 nm; this decreased to 0. UV absorbance detection. M2. Ensure data accuracy of your reader. It also integrates with the. The plate reader consisted of a large-area illumination unit and a parallel detection unit; see Fig. 340 PC384. 384-Well Microarray/High Sample Recovery Plate. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 5 fg per well to 0. Top. flat meniscus), but is approx 15 mL. REF (SpectraMax Plus 384 only) A reading of buffer, water, or air taken in the cuvette that is used as I 0 to calculate Absorbance or % Transmittance. For more sample throughput, the system canPlate Background OD, which was used for subsequent tests, was measured as follows. Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. Application Area: Multi mode plate reader. Dynamic range > 6 decades . For biochemical assays, the washer removes unbound material and unreacted reagents. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. 1. 96-384. 8 mm distance between well bottoms to plate bottomValidated by Molecular Devices for the FLIPR reader. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical. Browse for different fluorescent readers below and compare across different instrument suppliers. 2. Service Contracts, Preventative Maintenance and. If you have an xml plate object, then. 2. In general microplate washers are designed to leave only 2 microliters of residue per well and operate with an accuracy of 3% CV per well and a uniformity of 4% CV across a plate. Expanded wavelength coverageIn the software select Tools > Configuration. Ensure your assay quality with quality tips Certified RNase-/DNase-free Nonpyrogenic Nonsterile 50 racks/case. SpectraMax M Series Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. (or plate reader). Key benefits • Assess cell confluence objectively and quantitatively. Microseal 384-Well PCR Plates. A control well was set up to verify. 5in (46. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. 76 L x 85. Biotek Synergy 2 Plate Reader Multi-Mode. 130009WThe SpectraMax Paradigm reader with HTRF Detection Cartridge offers high-throughput screening capability with excellent read times and Z’ factors. the use of a reader calibrated with primary NIST and NMI standards. 0. SpectraMax ID5 Plate Reader (Molecular Devices) Description Internal price (Cornell and Cornell affiliates) External Price; SpectraMax ID5 Plate Reader Use: $20/hr: $32/hr. 96-, 384-, and 1536. M2. SpectraMax iD5. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. +1 800. 384-well microplate compatible Well read area range: H7 to K22 Figure 1-3 24-Well Micro-Volume Sample Slide Figure 1-4. and the A1 corner of the plate adapter (Figure 1-2). SpectraMax® Plus 384 provides ultrafast, full spectral range. Sensitivity and dynamic range are demonstrated by the high Z’ factors obtained on the SpectraMax. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. x. All stand-alone instrument functions can be controlled using the software. Efficiency. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate despite. Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. Well scans from the Gemini XPS Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM /Ethidium Homodimer-1) assay kit. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. With optimized reagents, option to operate the. 96-deep-round-well-plate, 384-deep-square-well-plate, 24-deep-well-plates. Figure 6. In these readers, RLUs are proportional to counts/sec which doesn't change with integration time assuming the.